Bastyan Dam, located at Lake Rosebery in Tullah, is the shooting location for the death scene on the high bridge at the end of the film. It is a composite location as CGI and cutaways are used to create a sense of space that is unrealistic in relation to the actual rail bridge.

At the beginning of the film aerial shots show the characters travelling by car along Lake Plimsoll toward the Pieman River.

Mackintosh Dam is the shooting location of the misty scene early in the film where the characters travel by boat through a graveyard of sunken trees in search of the Tasmanian Tiger.

The Fatman Barge at the Pieman River crossing near Corinna and historic gravesites on the south riverbank feature in Dying Breed as the travellers visit the backwater hillbilly town called Sarah (named after Sarah Island, from which the cannibal convict Alexander Pearce escaped).

At the beginning of the film, when Nina and Matt fly to Australia and meet up with Jack for the first time, they have a beer and plan the expedition in search of the Tasmanian Tiger. While the scene is set in Hobart, it is shot at the Water Rat Hotel in Melbourne.

Dying Breed features a fiction township called 'Sarah', located in Tasmania's South-West.

Murchison Dam is the shooting location for the scene where the characters have gone too far in their boat, overshooting the mark as they search for the Tasmanian Tiger. They reach the spillway and have to turn back. Behind them a mysterious figure watches from the mist on the spillway.

The Zeehan Spray Tunnel is the location the protagonists travel through to get to the wilderness hut where Nathan Phillips's character gets caught in the man trap.